GB 4789.40-2016 Translated English of Chinese Standard. GB4789.40-2016: National Food Safety Standard - Food Microbiological Examination - Examination of Cronobacter (Enterobacter Sakazakii)This Standard specifies the examination method for Cronobacter in food. This Standard applies to the examination of Cronobacter in infant formula, milk and dairy products and their raw materials. |
Contents
3 | |
Scope | 4 |
Medium and reagent | 5 |
Operation steps | 6 |
Result and report | 7 |
Operation steps | 8 |
Annex A Medium and reagent | 9 |
Annex B The most probable number MPN search table for Cronobacter | 14 |
Common terms and phrases
0.015 g Distilled 1.0 g Bromocresol 3.0 g Glucose 5.0 g Yeast add into 900 adjust pH Agar amygdalin Autoclave at 121°C Bromocresol purple 0.015 Buffer peptone water chloride 5.0 g Culture at 30°C Culture at 36°C D-sucrose decarboxylase test Dipotassium phosphate dissolve the components Distilled water 1000 Examination of Cronobacter Experimental method Inoculate extract 3.0 g Food Microbiological Examination g Bromocresol purple g Dipotassium g mL g Potassium dihydrogen g Sodium g Yeast extract Glucose 1.0 Glucose 1.0 g Heat and dissolve Inoculate the culture L-lysine decarboxylase medium L-ornithine decarboxylase medium lauryl sulfate tryptose liquid level liquid medium medium is purple mL of BPW mLST-Vm broth Modified lauryl sulfate monohydrochloride 5.0 negative is yellow Observe the results oxidase test phosphate 1.0 g phosphate 2.75 g pipette Potassium dihydrogen phosphate preheated to 44°C Preparation method Heat Sodium chloride 5.0 Sub-packaging 5 mL test is positive Tryptone tube vancomycin solution Yeast extract 3.0